Francesco Di Serio

Viroids and viroid diseases

Viroids are the smallest infectious agents known so far. Consisting of a small circular RNA, they may cause severe diseases to economically relevant crops (tomato, potato, peach, citrus, etc.). In contrast to viruses, viroids do not code for any protein. Therefore, the infectious process relies on the presence of structural domains in the viroid RNAs allowing them to interact directly with host factors. This feature makes viroids useful implements for getting deeper insights into the structural-functional relationships of both foreign and host RNAs.

In our laboratory, we develop multidisciplinary researches – also based on high-throughput sequencing technologies – addressed to further characterize molecular bases underlying plant-viroid interaction, with major interest in unveiling the role of RNA silencing in viroid trafficking and pathogenesis.

From an applied point of view, we focus on molecular and biological characterization viroids causing diseases in fruit trees, grapevine, vegetables and ornamental plants, on development of fast and reliable detection methods, and on the identification of control strategies of viroid diseases.

Publications

  • 2013 - Cytopathic effects incited by viroid RNAs and putative underlying mechanisms - Di Serio, F., De Stradis, A., Delgado, S., Flores, R. & Navarro Ramirez B. - Frontiers in plant science, 1-13
  • 2013 - The genetic diversity of Citrus dwarfing viroid populations is mainly dependent on the infected host species - Tessitori, M., Rizza, S., Reina, A., Causarano, G. & Di Serio, F. - Journal of general virology, 687-693
  • 2012 - Development And Validation Of A Multiplex Rt-pcr Method For The Simultaneous Detection Of Five Grapevine Viroids - Hajizadeh M.1, Navarro B., Bashir N. S.1, Torchetti E.m., Di Serio F. - Journal Of Virological Methods (03232J0), volume 179 pag. 62-69
  • 2012 - First report of Chrysanthemum stunt viroid in Argyranthemum frutescens in Italy - Torchetti E.M., Navarro B., Trisciuzzi V.N., Nuccitelli L., Silletti M.R. & Di Serio F. - Journal of plant pathology, 451-454
  • 2012 - Viroids: How to infect a host and cause disease without encoding proteins - Navarro, B., Gisel, A., Rodio, M.E., Delgado, S., Flores, R. & Di Serio, F. - Biochimie, 1474-1480
  • 2012 - A single polyprobe for detecting simultaneously eight pospiviroids infecting ornamentals and vegetables - Torchetti, E.M., Navarro, B. & Di Serio, F. - Journal of virological methods, 141-146
  • 2012 - The role of plant viroids in diseases – new developments - Di Serio, F., Torchetti, E.M., Flores, R. & Navarro, B. - Perspectives in agriculture, veterinary science, nutrition and natural resources, 1-9
  • 2012 - Small RNAs containing the pathogenic determinant of a chloroplast-replicating viroid guide the degradation of a host mRNA as predicted by RNA silencing - Navarro, B., Gisel, A., Rodio, M.E., Delgado, S., Flores, R. & Di Serio, F. - Plant journal, 991-1003
  • 2012 - Viroids: from genotype to phenotype just relying on RNA sequence and structural motifs - Flores, R., Serra, P., Minoia, S., Di Serio, F. & Navarro, B. - Frontiers in microbiology, 1-13
  • 2012 - I viroidi delle colture ornamentali: emergenze fitosanitarie e misure di controllo - Di Serio, F., Torchetti, E.M. & Navarro, B. - Criticità e prospettive delle emergenze fitosanitarie , 37-49
  • 2011 - Apple Dimple Fruit Viroid - Di Serio F, Malfitano M. 1, Alioto D. 1, Ragozzino A. 1 & Flores R. - Virus And Virus-like Diseases Of Pome And Stone Fruits , Publisher: Hadidi A., Barba M., Candresse T., Jelkmann W. (AP
  • 2011 - Viroids - Owens R.a.1, Flores R. 2, Di Serio, F., Li S-f. 3, Pallas V. 4, Randles J.w. 5, Sano T. 6 & Vidalakis G. 7 - Virus Taxonomy: Ninth Report Of The International Committee On Taxonomy Of Viruses , Publisher: (Eds: Andrew M.Q. King, Michael J. Adams, Eric B.
  • 2011 - Viroids And Viroid Diseases Of Plants - Flores R. (1), Di Serio F., Navarro Ramirez B., Duran-vila N. (2) & Owens R.a. (3) - Studies In Viral Ecology, Volume One: Microbial And Botanical Host Systems , Publisher: Edited by Christon J. Hurst , John Wiley & Sons, I
  • 2011 - Peach Latent Mosaic Viroid And Its Diseases In Peach Trees - Flores R.1, Di Serio F., Hernandez C. 1, Barba M. 2 & Hadidi A.3 - Virus And Virus-like Diseases Of Pome And Stone Fruits , Publisher: Hadidi A., Barba M., Candresse T., Jelkmann W. (AP
  • 2011 - Somatic Embryogenesis Efficiently Eliminates Viroid Infections From Grapevines - Gambino G., Navarro Ramirez B. , Vallania R., Gribaudo I. & Di Serio F. - European Journal Of Plant Pathology (10845J0), volume 130 pag. 511-519
  • 2011 - Citrus Tristeza Virus Infection Induces The Accumulation Of Viral Small Rnas (21–24-nt) Mapping Preferentially At The 3’-terminal Region Of The Genomic Rna And Affects The Host Small Rna Profile - Ruiz-ruiz S. (1), Navarro Ramirez B., Gisel A.,peÑa L (2)• Navarro L. (2), Moreno P. (2), Di Serio F. & •flores R. (1) - Plant Molecular Biology (05842J0), volume 75 pag. 607-619
  • 2011 - Viroids - R. Flores1, J.a. Daros1, Hernandez C.1& Di Serio F. - Encyclopedia Of Life Sciences , Publisher: John Wiley & Sons, Inc
  • 2010 - Detection And Identification Of Viroids Damaging Potato And Fruit Trees - Di Serio F. & Navarro Ramirez B. - Élelmiszerbiztonság Megítélési Módszerei Ii (Methods to evaluate food safety. II), Publisher: Editor: Arpad Ambrus. Publishing: Edison House Hol
  • 2010 - Viroidi: Una Classe Di Patogeni Emergenti Poco Nota - Di Serio F., Navarro R.b. - Protezione Delle Colture , volume III-4 pag. 2-8
  • 2010 - Multiple Viroid Infections In Iranian Grapevines - Hajizadeh M. (1), Navarro B. Bashir N.s.(1) & Di Serio F. - Journal Of Plant Pathology (00077NN), Publisher: 92
  • 2010 - Virosi E Fitoplasmosi - Barba M.(1), Martelli G.p.(2), Tomassoli L.(1), Gallitelli D.(2), Di Serio F. & Pasquini G.(1) - Il Pomodoro pp. 220-235 (ed. V. Magnifico), Publisher: Bayer CropScience
  • 2010 - Rna-dependent Rna Polymerase 6 Delays Accumulation And Precludes Meristem Invasion Of A Nuclear-replicating Viroid - Di Serio F., Martínez De Alba Ae, Navarro B, Gisel A, Flores R. - Journal Of Virology (62788J0), volume 84 pag. 2477-2489
  • 2010 - Molecular Characterization Of Turkish Isolates Of Pear Blister Canker Viroid And Assessment Of The Sequence Varaibility Of This Viroid - Yesilcollou S., Minoia S., Torchetti E.m , S. Kaymak S., Gumus M., Myrta A., Navarro B., Di Serio F. - Journal Of Plant Pathology (00077NN), volume 92 pag. 813-819
  • 2009 - Multiple Viroid Infections In Iranian Grapevines - Hajizadeh M. (1), Navarro Ramirez, B. Bashir N.s.(1) & Di Serio F. - Journal Of Plant Pathology (00077NN), Publisher: 92(4)
  • 2009 - Il Viroide Pstvd Minaccia Pomodoro E Patata - Silletti M.r., Navarro B., Bozzano G., Trisciuzzi V.n., Di Serio F - Informatore Agrario , volume 11 pag. 89-90
  • 2009 - Deep Sequencing Of Viroid-derived Small Rnas From Grapevine Provides New Insights On The Role Of Rna Silencing In Plant-viroid Interaction. - Navarro, B., Pantaleo, V., Gisel, A., Moxon, S., Dalmay, T., Bisztray, G., Di Serio, F., Burgyan, J. - Plos One (00098NN), Publisher: 4
  • 2009 - Deep Sequencing Of The Small Rnas Derived From Two Symptomatic Variants Of A Chloroplastic Viroid: Implications For Their Genesis And For Viroid Pathogenesis - Di Serio F., Gisel A., Navarro B., Delgado S., Martínez De Alba Á.e., Donvito G. And Flores R. - Plos One (00098NN), Publisher: 4
  • 2009 - Identification And Characterization Of Potato Spindle Tuber Viroid Infecting Tomato In Italy - Navarro, B., Silletti, M.r., Trisciuzzi, V.n., Di Serio, F. - Journal Of Plant Pathology (00077NN), volume 91 pag. 723-726
  • 2008 - Sequences Of The Smallest Double-stranded Rnas Associated With Cherry Chlorotic Rusty Spot And Amasya Cherry Diseases. - Covelli L., Kozlakidis Z., Di Serio F., Citir A., Aç1kgöz S., Hernández C., Ragozzino A., Coutts R.h. And R. Flores - Archives Of Virology (15471J0), volume 153 pag. 759-762
  • 2008 - Viroid-host Interactions: A Molecular Dialogue Between Two Uneven Partners - Flores, R., Carbonell, A., Gago, S., Martinez De Alba, A.e., Delgado, S., Rodio, M.e. & Di Serio, F. - Biology Of Plant Microbe Interactions Cd, Vol. 6 , Publisher: Lorito, M., Woo, S.L. Scala, F.
  • 2008 - Viroids: Molecular Implements For Dissecting Rna Trafficking In Plants. - Di Serio, F., Flores, R. - Rna Biology (00199NN), volume 5 pag. x-x
  • 2008 - First Report Of Cucumber Mosaic Virus Infecting Solanum Jasminoides In Italy. - Davino, S., Di Serio, F., Polizzi, G., Tessitori, M. - Plant Disease (01997J0), Publisher: 92
  • 2008 - Molecular And Developmental Alterations Elicited By A Chloroplastic Viroid - Di Serio F., Rodio M.e., Delgado S., De Stradis A., Gómez M.d, Flores R. - Biology Of Plant Microbe Interaction Cd, Vol. 6 , Publisher: Lorito, M., Woo, S.L., Scala, F.
  • 2007 - First Report Of Pear Blister Canker Viroid In Malta - (1)attard A., (2)afechtal M., (1)agius M., (3)matic S., (2)gatt M., (2)myrta A., Di Serio F. - Journal Of Plant Pathology (00077NN), Publisher: 89
  • 2007 - First Report Of Apple Dimple Fruit Viroid In Lebanon. - Choueiri E., El Zammar S., Jreijiri F., Hobeika C., (1)myrta A., Di Serio F. - Journal Of Plant Pathology (00077NN), volume 89 p. 304
  • 2007 - A Viroid Rna With A Specific Structural Motif Inhibits Chloroplast Development - (1) Rodio M.e., (2) Delgado S., De Stradis A., (2)gómez M.d., (2)flores R., Di Serio F. - Plant Cell (09000J0), volume 19 pag. 3610-3616
  • 2007 - Identification And Characterization Of Potato Spindle Tuber Viroid Infecting Solanum Jasminoides And S. Rantonnetii In Italy. - Di Serio, F. - Journal Of Plant Pathology (00077NN), volume 89 pag. 297-300
  • 2007 - Detection By Tissue-printing Of Pome Fruit Viroids And Charaterization Of Pear Blister Canker Viroid In Bosnia And Herzegovina. - (1) Lolic B., (2)afechtal M., (1)matic S., (2)myrta A., Di Serio F. - Journal Of Plant Pathology (00077NN), volume 89 pag. 369-375
  • 2007 - Diagnosi Del Viroide Del Tubero Fusiforme Della Patata In Solanacee Ornamentali. - Di Serio F., Silletti M.r., Trisciuzzi V.n., Guario A., Percoco A., Lillo A.r. - Informatore Fitopatologico , volume 57(12 pag. 82-85
  • 2006 - Variants Of Peach Latent Mosaic Viroid Inducing Peach Calico: Uneven Distribution In Infected Plants And Requirements Of The Insertion Containing The Pathogenicity Determinant. - Rodio, M.e., Delgados, S., Flores, R. & Di Serio, F. - Journal Of General Virology (54890J0), volume 87 pag. 231-240
  • 2006 - Peach Latent Mosaic Viroid: Not So Latent - Flores, R. (1), Delgado, S. (1), Rodio, M.e. (2), Ambros, S. (3), Hernandez, C. (1), Di Serio, F. (2) - Molecular Plant Pathology (13843J0), volume 7 pag. 209-222
  • 2006 - Molecular Characterization Of The Largest Mycoviral-like Double-stranded Rnas Associated With Amasya Cherry Disease, A Disease Of Presumed Fungal Aetiology. - Kozlakidis, Z., Covelli, L., Di Serio, F., Citir, A., Acikgoz, S., Hernandez, C., Ragozzino, A., Flores, R & Coutts, R.h.a. - Journal of General Virology (15471J0), volume 87 pp. 3113-3117
  • 2006 - Close Structural Relationships Between Two Hammerhead Viroid-like Rnas Associated With Cherry Chlorotic Rusty Spot Disease. - Di Serio, F., Daros, J.a., Ragozzino, A. & Flores, R. - Archives Of Virology (15471J0), volume 151 pag. 1539-1549
  • 2005 - La Malattia Delle Macchie Clorotico-rugginose Del Ciliegio: Una Fitopatia Ad Eziologia Complessa Osservata Nei Ciliegeti Campani. - Alioto D., Covelli L., Zaccaria F., Di Serio F., Vitale F., Ragozzino A., - Informatore Fitopatologico , volume 3 pp. 45-51
  • 2004 - Cherry Chlorotic Rusty Spot And Amasya Cherry Diseases Are Associated With A Complex Pattern Of Mycoviral-like Double-stranded Rnas. I. Characterization Of A New Species In The Genus Chrysovirus. - Covelli L, Coutts Rh, Di Serio F, Citir A, Açikgöz S, Hernández C, Ragozzino A, Flores R. - Journal Of General Virology (54890J0), volume 85 pag. 3389-3397
  • 2004 - Experimental Evidence That Apple Dimple Fruit Viroid Does Not Spread Naturally.. - Malfitano M., Alioto D., Di Serio F., Ragozzino A., Flores R. - Acta Horticulturae , volume 657 pag. 367-371
  • 2004 - Cherry Chlorotic Rusty Spot And Amasya Cherry Disease, Two Names For A Single Disease Associated With A Complex Pattern Of Mycoviral-like Double-stranded Rnas. Ii. Characterization Of A New Species Of Genus Partitivirus. - Coutts R.h.a., Covelli L, Di Serio F., Citir A., Aç1kgöz S., Hernández C., Ragozzino A., And Flores R. - Journal Of General Virology (54890J0), volume 85 pag. 3399-3403
  • 2003 - Viroids Of Stone Fruits: Incidence And Diseases In The Mediterranean - Pallas V., Amari K., Gomez G., Abou Ghanem-Sabanadzovic N. & Di Serio F. - Options Méditerranéenes, Series B, volume 45 pp. 129-133
  • 2003 - Light And Electron Microscope Observations On Chlorotic Rusty Spot, A Disorder Of Cherry In Italy. - Alioto D., Zaccaria F., Covelli L., Di Serio F., Ragozzino A., Milne R.g. - Journal Of Plant Pathology (00077NN), volume 85 pag. 215-218
  • 2003 - First Report Of Peach Latent Mosaic Viroid And Hop Stunt Viroid Infecting Peach Trees In The Czech. Republic. - Hassan M., Rysanek P., Di Serio F. - Plant Disease (01997J0), volume 87 pag. 1537--
  • 2003 - Viroid-like Satellite Rnas - Rubino L. , Di Serio F. , Martelli G.P. - Viroids, Hadidi, A., Flores, R., Randles, J., & Semancik, Publisher: CSIRO Publishing, pp. 76-84
  • 2003 - Peach Latent Mosaic Viroid Variants Inducing Peach Calico (extreme Chlorosis) Contain A Characteristic Insertion That Is Responsable For This Symptomatology. - Malfitano M.1, Di Serio F. 2, Covelli L., 3, Ragozzino A. 4, Hernandez C. 5, Flores R. 6. - Virology (95304J0), volume 313 pag. 429-501
  • 2003 - Apple Dimple Fruit Viroid - Di Serio F., Malfitano M., Alioto D., Ragozzino A., Flores R. - Viroids ( Eds. Hadidi, A., Flores, R., Randles, J.W. & Semancik, J.S.)., Publisher: Csiro publishing, pp. 146-150
  • 2002 - Homology-dependent Virus Resistance Against Cymbidium Ringspot Virus Is Inhibited By Post-transcriptional Gene Silencing Suppressor Viruses - Di Serio F., Rubino L., Russo M., Martelli G.p. - Journal Of Plant Pathology , Publisher: 84
  • 2002 - Apple Dimple Fruit Viroid: Sequence Variability And Its Specific Detection By Fluorescent Rt-pcr In The Presence Of Apple Scar Skin Viroid - Di Serio F., Malfitano M., Alioto D., Ragozzino A., Flores R - Journal Of Plant Pathology , Publisher: 84
  • 2001 - Local Expression Of Enzymatically Active Class I Beta-1, 3-glucanase Enhances Symptoms Of Tmv Infection In Tobacco. - Bucher G.l.1, Tarina C.2, Heinlein M.3, Di Serio F.4, Meins F. Jr 5, Iglesias V.a.6 - Plant Journal (09659J0), volume 28 pag. 361-369
  • 2001 - Sense- And Antisense-mediated Gene Silencing In Tobacco Is Inhibited By The Same Viral Suppressors And Is Associated With Accumulation Of Small Rnas - Di Serio F. 1, Schob H. 2, Iglesias A. 3, Tarina C. 4, Bouldoires E. 5, Meins F. 6. - Psychologie-schweizerische Zeitschrift Fur Psychologie Und Ihre Andwendungen (81559J0), volume 98 pag. 6506-6510