Giorgio Gambino

Trasformazione genetica e caratterizzazione molecolare di piante transgeniche di vite, tabacco ed Arabidopsis per la resistenza a virus e per analisi funzionali.

Induzione di embriogenesi somatica a partire da vari tessuti di vite e studio dei meccanismi molecolari alla base del processo (geni WOX).

Studio di microRNA (miRNA) e small interfering RNA (siRNA) in vite.

Tecniche di ibridazione in situ per il rilevamento di acidi nucleici virali e mRNA di vite.

Diagnosi dei principali virus, viroidi e fitoplasmi che colpiscono la vite attraverso metodologie sierologiche e molecolari.

Utilizzo e confronto di varie tecniche di risanamento applicate a cloni di vite.

Studio delle interazioni virus-vite mediante approcci molecolari.

Progetti

Pubblicazioni

  • 2014 - Functional genomics in fruit trees - Gambino G., Pagliarani C., Gribaudo I. - Tree Biotechnology, 583-613
  • 2013 - Genome-wide identification of viral and host transcripts targeted by viral siRNAs in Vitis vinifera - Miozzi L., Gambino G., Burgyan J. & Pantaleo V. - Molecular plant pathology, 30-43
  • 2013 - Hydrogen Peroxide Accumulation and Transcriptional Changes in Grapevines Recovered from Flavescence Dorée Disease - Gambino G., Boccacci P., Margaria P., Palmano S., Gribaudo I. - Phytopathology, 103:776-784
  • 2012 - The grapevine root-specific aquaporin VvPIP2;4N controls root hydraulic conductance and leaf gas exchange under well watered conditions but not under water stress - Perrone I., Gambino G., Chitarra W. Vitali M., Pagliarani C., Riccomagno N., Balestrini R., Kaldenhoff R., Uehlein N., Gribaudo I., Schubert A., Lovisolo C. - Plant Physiology , 965-977
  • 2012 - Co-evolution between Grapevine rupestris stem pitting associated virus and Vitis vinifera L. leads to decreased defence responses and increased transcription of genes related to photosynthesis - Gambino, G., Cuozzo D., Fasoli, M., Pagliarani, C., Vitali, M., Boccacci, P., Pezzotti M. & Mannini F. - Journal of experimental botany, 5919-5933
  • 2012 - Genetic transformation of fruit trees: current status and remaining challenges - Gambino, G. & Gribaudo, I. - Transgenic research, 1163-1181
  • 2011 - Functional Effect Of Grapevine 1-deoxy-d-xylulose 5-phosphate Synthase Substitution K284n On Muscat Flavour Formation - Battilana J.1, Emanuelli F. 1, Gambino G., Gribaudo I., Gasperi F. 2, Boss P 3. & Grando M.s 1 - Journal Of Experimental Botany (53988J0), volume 62 pag. 5497-5508
  • 2011 - Characterization Of Expression Dynamics Of Wox Homeodomain Transcription Factors During Somatic Embryogenesis In Vitis Vinifera. - Gambino G., Minuto M. (1), Boccacci P. (1), Perrone I. (1), Vallania R., Gribaudo I. - Journal Of Experimental Botany (53988J0), volume 62 pag. 1089-1101
  • 2011 - Somatic Embryogenesis Efficiently Eliminates Viroid Infections From Grapevines - Gambino G., Navarro Ramirez B. , Vallania R., Gribaudo I. & Di Serio F. - European Journal Of Plant Pathology (10845J0), volume 130 pag. 511-519
  • 2011 - Non-coding Rnas And Gene Silencing In Grape - Carra A. 1, Gambino G., Urso S 2. & Nervo G. 1 - Non Coding Rnas In Plants, Rna Technologies , Publisher: Springer-Verlag
  • 2010 - Transgene Silencing In Grapevines Transformed With GFLV Resistance Genes: Analysis Of Variable Expression Of Transgene, siRNA Production And Cytosine Methylation. - Gambino G., Perrone I., Carra A., Chitarra W., Boccacci P., Torello Marinoni D., Barberis M., Maghuly F., Laimer M., Gribaudo I. - Transgenic Research (09931J0), volume 19 pag. 17-27
  • 2010 - Small Rna Extraction And Expression Analysis By Northern Blot. - Batelli G. (1), Gambino G., Mica E. (2), Schubert A.(1), Carra A.(1) - In: Methodologies And Results In Grapevine Research, S. Delrot Et Al (eds), Publisher: Springer Science+Business Media B.V., Dordrecht
  • 2010 - Viplasmi: Tecniche Di Riduzione Del Danno: Termoterapia In Acqua Del Materiale Di Moltiplicazione Viticolo - Mannini F., Gribaudo I.,m Cuozzo D., Gambino G. - Quaderni Della Regione Piemonte - Agricoltura , volume 72 pag. 203-207
  • 2010 - Transgene Silencing In Plants: Mechanisms, Applications And New Perspectives - Pagliarani C., Perrone I., Carra A., Gambino G. - In: Gene Silencing: Theory, Techniques And Applications, A.j. Catalano (eds), Publisher: Nova Science Publishers , NY, USA
  • 2010 - In Situ Localization Of Grapevine Fanleaf Virus And Phloem-restricted Viruses In Embryogenic Callus Of Vitis Vinifera - Gambino, G., Vallania, R., Gribaudo, I. - European Journal Of Plant Pathology (10845J0), volume 127 pag. 557-570
  • 2010 - Field Assessment And Diagnostic Methods For Detection Of Grapevine Viruses. - Gambino G., Angelini E.(1), Gribaudo I. - In: Methodologies And Results In Grapevine Research, S. Delrot Et Al (eds), Publisher: Springer Science+Business Media B.V., Dordrecht
  • 2010 - Virit: Monitoraggio Della Diffusione Di Reinfezioni Virali Dopo L’impianto Del Vigneto Con Viti Risanate. Virusvite: Diffusione Di Reinfezioni Nel Vigneto E Ruolo Delle Cocciniglie Vettrici Di Virus - Gribaudo I., Mannini F., Gambino G., Cuozzo D., Argamante N, Bosco D. 1, Bertin S. 1, Della Valle D. 2, Cotroneo A. 3 - Quaderni Della Regione Piemonte - Agricoltura , volume 72 pag. 209-212
  • 2009 - Monitoring The Spread Of Viruses After Vineyard Replanting With Heat-treated Clones Of Vitis Vinifera ‘nebbiolo’ - Gribaudo, I., Gambino, G., Bertini, S., Bosco, D., Cotroneo, A., Mannini, F. - Journal Of Plant Pathology (00077NN), volume 91 pag. 633-636
  • 2009 - Elimination Of Grapevine Fanleaf Virus From Three Vitis Vinifera Cultivars By Somatic Embryogenesis - Gambino, G., Di Matteo, D. , Gribaudo, I. - European Journal Of Plant Pathology (10845J0), volume 123 pag. 57-60
  • 2009 - Characterization Of T-dna Insertions In Transgenic Grapevines Obtained By Agrobacterium-mediated Transformation. - Gambino, G., Chitarra, W., Maghuly, F., Laimer, M., Boccacci, P., Torello Marinoni, D., Gribaudo, I,. - Molecular Breeding (11451J0), volume 24 pag. 305-320
  • 2009 - Molecular Characterization Of Wild Grape Populations From North Western Italy And Their Genetic Relationship With Cultivated Varieties - Schneider, A., Torello Marinoni, D., Raimondi, S., Boccacci, P., Gambino, G. - Acta Horticulturae , volume 827 pag. 211-216
  • 2009 - Assessment Of Genetic Fidelity In Regenerants From Two Vitis Vinifera Cultivars - Gribaudo, I., Torello Marinoni, D., Gambino, G., Mannini, F., Akkak, A., Botta, R. - Acta Horticulturae , volume 827 pag. 131-135
  • 2009 - Cloning And Characterization Of Small Non-coding Rnas From Grape - Carra, A., Mica, E., Gambino, G., Pindo, M., Maser, C., Pe, E. , Schubert, A. - Plant Journal (09659J0), volume 59 pag. 750-763
  • 2009 - Application Of A Site-specific Dna Excision Strategy For Marker Gene Removal During Gene Transfer In Vitis Spp. - Martinellii, L., Dalla Costa, L., Vaccari, I., Poletti, V., Gribaudo,i., Gambino, G., Guzzo, F., Saldarelli, P., Minafra, A., Turturo,c. - Acta Horticulturae , volume 827 pag. 399-403
  • 2008 - Viti Sane E Risanate Unica Lotta Alle Virosi. - Gribaudo, I., Mannini, F., Cuozzo, D., Gambino, G., Argamante, N., Bertin, S., Bosco, D., Cotroneo, A. - Informatore Agrario , volume 46 pag. 59-61
  • 2008 - A Rapid And Effective Method For Rna Extraction From Different Tissues Of Grapevine And Other Woody Plants. - Gambino, G., Perrone, I., Gribaudo, I. - Phytochemical Analysis (00205NN), volume 19 pag. 520-522
  • 2007 - A Cetyltrimethylammonim Bromide-based Method To Extract Low-molecular-weight Rna From Polysaccharide-rich Plant Tissues. - Carra A., Gambino G., Schubert A. - Analytical Biochemistry (09108J0), volume 360 pag. 318-320
  • 2007 - Somatic Embryogenesis From Whole Flowers, Anthers And Ovaries Of Grapevine (vitis Spp.). - Gambino G., (1)Ruffa P., Vallania R., Gribaudo I. - Plant Cell Tissue And Organ Culture (04987J0), volume 90(1) pag. 79-83
  • 2006 - Simultaneous Detection Of Nine Grapevine Viruses By Multiplex Reverse Transcriptionpolymerase Chain Reaction With Coamplification Of A Plant Rna As Internal Control. - Gambino, G. & Gribaudo, I. - Phytopathology (77147J0), volume 96 pag. 1223-1229
  • 2006 - Molecular Characterization Of Grapevine Plants Transformed With GFLV Resistance Genes: II. - Maghuli F., Leopold S., Da Camara Machado A., Borroto Fernandez E.G., Khan M.A., Gambino G., Gribaudo I., Laimer M. - Plant Cell Reports (06015J0), volume 25 pag. 546-553
  • 2006 - Detection And Elimination Of Viruses In Callus, Somatic Embryos And Regenerated Plantlets Of Grapevine. - Gambino, G., Bondaz, J. & Gribaudo, I. - European Journal Of Plant Pathology (10845J0), volume 114 pag. 397-404
  • 2005 - Molecular Characterization Of Grapevine Plants Transformed With GFLV Resistance Genes: I. - Gambino G., Gribaudo I., Leopold S., Schartl A., Laimer M. - Plant Cell Reports (06015J0), volume 24 pag. 655-662
  • 2005 - Genetic Characterization Of Endangered Grape Cultivars Of Reggio Emilia Province. - Boccacci P., Torello Marinoni D., Gambino G., Botta R., Schneider A. - American Journal Of Enology And Viticulture (06974J0), volume 56(4) pag. 411-416
  • 2005 - Molecular Characterization Of Transgenic Grapevine Plants - Gribaudo I., Gambino G., Leopold S., Laimer M. - Acta Horticulturae , volume 689 pag. 485-492
  • 2004 - Somatic Embryogenesis From Grapevine Anthers: The Optimal Developmental Stage For Collecting Explants. - Gribaudo I., Gambino G., Vallania R. - American Journal Of Enology And Viticulture (06974J0), volume 55 pag. 427-430
  • 2004 - Risanamento Di Cloni Di Vite (vitis Vinifera L.) Da Virus Floematici Mediante Embriogenesi Somatica - Bondaz J., Gambino G., Cuozzo D., Gribaudo I. - Petria , volume 14 pag. 99-108
  • 2004 - Cryopreservation Of Vitis Vinifera L. Somatic Embryos By Vitrification Or Encapsulation-dehydration. - Miaja M.l., Vallania R., Gribaudo I., Gambino G. - Acta Horticulturae , volume 663 pag. 599-603
  • 2003 - Ovary As Valuable Explant For Somatic Embryogenesis Induction In Grapes (vitis Spp.) - Martinelli L., Gribaudo I. Semenzato M., Poletti V., Gambino G. - Acta Horticulturae , volume 603 pag. 499-504
  • 2003 - Transformation Of Vitis Vinifera L. Cv Nebbiolo With The Coat Protein Gene Of Grapevine Fanleaf Virus (gflv). - Gribaudo I., Scariot V., Gambino G., Schubert A., Golles R., Laimer M. - Acta Horticulturae , volume 603 pag. 309-314