Giorgio Gambino

Genetic transformation and molecular characterization  of transgenic plants of grapevine, tobacco and Arabidopsis for virus resistance and for functional studies.

In vitro culture, induction of somatic embryogenesis in grapevine, and study on molecular bases of embryogenesis (WOX genes).

Isolation of small RNA from grapevine and study on microRNA (miRNA) and small interfering RNA (siRNAs).

In situ hybridization for virus detection and gene expression analyses.

Detection of viruses, viroids and phytoplasma by serological and molecular techniques.

Virus and viroid eradication in grapevine.

Molecular interactions between viruses and grapevine.

Projects

Publications

  • 2014 - Functional genomics in fruit trees - Gambino G., Pagliarani C., Gribaudo I. - Tree Biotechnology, 583-613
  • 2013 - Genome-wide identification of viral and host transcripts targeted by viral siRNAs in Vitis vinifera - Miozzi L., Gambino G., Burgyan J. & Pantaleo V. - Molecular plant pathology, 30-43
  • 2013 - Hydrogen Peroxide Accumulation and Transcriptional Changes in Grapevines Recovered from Flavescence Dorée Disease - Gambino G., Boccacci P., Margaria P., Palmano S., Gribaudo I. - Phytopathology, 103:776-784
  • 2012 - The grapevine root-specific aquaporin VvPIP2;4N controls root hydraulic conductance and leaf gas exchange under well watered conditions but not under water stress - Perrone I., Gambino G., Chitarra W. Vitali M., Pagliarani C., Riccomagno N., Balestrini R., Kaldenhoff R., Uehlein N., Gribaudo I., Schubert A., Lovisolo C. - Plant Physiology , 965-977
  • 2012 - Co-evolution between Grapevine rupestris stem pitting associated virus and Vitis vinifera L. leads to decreased defence responses and increased transcription of genes related to photosynthesis - Gambino, G., Cuozzo D., Fasoli, M., Pagliarani, C., Vitali, M., Boccacci, P., Pezzotti M. & Mannini F. - Journal of experimental botany, 5919-5933
  • 2012 - Genetic transformation of fruit trees: current status and remaining challenges - Gambino, G. & Gribaudo, I. - Transgenic research, 1163-1181
  • 2011 - Functional Effect Of Grapevine 1-deoxy-d-xylulose 5-phosphate Synthase Substitution K284n On Muscat Flavour Formation - Battilana J.1, Emanuelli F. 1, Gambino G., Gribaudo I., Gasperi F. 2, Boss P 3. & Grando M.s 1 - Journal Of Experimental Botany (53988J0), volume 62 pag. 5497-5508
  • 2011 - Characterization Of Expression Dynamics Of Wox Homeodomain Transcription Factors During Somatic Embryogenesis In Vitis Vinifera. - Gambino G., Minuto M. (1), Boccacci P. (1), Perrone I. (1), Vallania R., Gribaudo I. - Journal Of Experimental Botany (53988J0), volume 62 pag. 1089-1101
  • 2011 - Somatic Embryogenesis Efficiently Eliminates Viroid Infections From Grapevines - Gambino G., Navarro Ramirez B. , Vallania R., Gribaudo I. & Di Serio F. - European Journal Of Plant Pathology (10845J0), volume 130 pag. 511-519
  • 2011 - Non-coding Rnas And Gene Silencing In Grape - Carra A. 1, Gambino G., Urso S 2. & Nervo G. 1 - Non Coding Rnas In Plants, Rna Technologies , Publisher: Springer-Verlag
  • 2010 - Transgene Silencing In Grapevines Transformed With GFLV Resistance Genes: Analysis Of Variable Expression Of Transgene, siRNA Production And Cytosine Methylation. - Gambino G., Perrone I., Carra A., Chitarra W., Boccacci P., Torello Marinoni D., Barberis M., Maghuly F., Laimer M., Gribaudo I. - Transgenic Research (09931J0), volume 19 pag. 17-27
  • 2010 - Small Rna Extraction And Expression Analysis By Northern Blot. - Batelli G. (1), Gambino G., Mica E. (2), Schubert A.(1), Carra A.(1) - In: Methodologies And Results In Grapevine Research, S. Delrot Et Al (eds), Publisher: Springer Science+Business Media B.V., Dordrecht
  • 2010 - Viplasmi: Tecniche Di Riduzione Del Danno: Termoterapia In Acqua Del Materiale Di Moltiplicazione Viticolo - Mannini F., Gribaudo I.,m Cuozzo D., Gambino G. - Quaderni Della Regione Piemonte - Agricoltura , volume 72 pag. 203-207
  • 2010 - Transgene Silencing In Plants: Mechanisms, Applications And New Perspectives - Pagliarani C., Perrone I., Carra A., Gambino G. - In: Gene Silencing: Theory, Techniques And Applications, A.j. Catalano (eds), Publisher: Nova Science Publishers , NY, USA
  • 2010 - In Situ Localization Of Grapevine Fanleaf Virus And Phloem-restricted Viruses In Embryogenic Callus Of Vitis Vinifera - Gambino, G., Vallania, R., Gribaudo, I. - European Journal Of Plant Pathology (10845J0), volume 127 pag. 557-570
  • 2010 - Field Assessment And Diagnostic Methods For Detection Of Grapevine Viruses. - Gambino G., Angelini E.(1), Gribaudo I. - In: Methodologies And Results In Grapevine Research, S. Delrot Et Al (eds), Publisher: Springer Science+Business Media B.V., Dordrecht
  • 2010 - Virit: Monitoraggio Della Diffusione Di Reinfezioni Virali Dopo L’impianto Del Vigneto Con Viti Risanate. Virusvite: Diffusione Di Reinfezioni Nel Vigneto E Ruolo Delle Cocciniglie Vettrici Di Virus - Gribaudo I., Mannini F., Gambino G., Cuozzo D., Argamante N, Bosco D. 1, Bertin S. 1, Della Valle D. 2, Cotroneo A. 3 - Quaderni Della Regione Piemonte - Agricoltura , volume 72 pag. 209-212
  • 2009 - Monitoring The Spread Of Viruses After Vineyard Replanting With Heat-treated Clones Of Vitis Vinifera ‘nebbiolo’ - Gribaudo, I., Gambino, G., Bertini, S., Bosco, D., Cotroneo, A., Mannini, F. - Journal Of Plant Pathology (00077NN), volume 91 pag. 633-636
  • 2009 - Elimination Of Grapevine Fanleaf Virus From Three Vitis Vinifera Cultivars By Somatic Embryogenesis - Gambino, G., Di Matteo, D. , Gribaudo, I. - European Journal Of Plant Pathology (10845J0), volume 123 pag. 57-60
  • 2009 - Characterization Of T-dna Insertions In Transgenic Grapevines Obtained By Agrobacterium-mediated Transformation. - Gambino, G., Chitarra, W., Maghuly, F., Laimer, M., Boccacci, P., Torello Marinoni, D., Gribaudo, I,. - Molecular Breeding (11451J0), volume 24 pag. 305-320
  • 2009 - Molecular Characterization Of Wild Grape Populations From North Western Italy And Their Genetic Relationship With Cultivated Varieties - Schneider, A., Torello Marinoni, D., Raimondi, S., Boccacci, P., Gambino, G. - Acta Horticulturae , volume 827 pag. 211-216
  • 2009 - Assessment Of Genetic Fidelity In Regenerants From Two Vitis Vinifera Cultivars - Gribaudo, I., Torello Marinoni, D., Gambino, G., Mannini, F., Akkak, A., Botta, R. - Acta Horticulturae , volume 827 pag. 131-135
  • 2009 - Cloning And Characterization Of Small Non-coding Rnas From Grape - Carra, A., Mica, E., Gambino, G., Pindo, M., Maser, C., Pe, E. , Schubert, A. - Plant Journal (09659J0), volume 59 pag. 750-763
  • 2009 - Application Of A Site-specific Dna Excision Strategy For Marker Gene Removal During Gene Transfer In Vitis Spp. - Martinellii, L., Dalla Costa, L., Vaccari, I., Poletti, V., Gribaudo,i., Gambino, G., Guzzo, F., Saldarelli, P., Minafra, A., Turturo,c. - Acta Horticulturae , volume 827 pag. 399-403
  • 2008 - Viti Sane E Risanate Unica Lotta Alle Virosi. - Gribaudo, I., Mannini, F., Cuozzo, D., Gambino, G., Argamante, N., Bertin, S., Bosco, D., Cotroneo, A. - Informatore Agrario , volume 46 pag. 59-61
  • 2008 - A Rapid And Effective Method For Rna Extraction From Different Tissues Of Grapevine And Other Woody Plants. - Gambino, G., Perrone, I., Gribaudo, I. - Phytochemical Analysis (00205NN), volume 19 pag. 520-522
  • 2007 - A Cetyltrimethylammonim Bromide-based Method To Extract Low-molecular-weight Rna From Polysaccharide-rich Plant Tissues. - Carra A., Gambino G., Schubert A. - Analytical Biochemistry (09108J0), volume 360 pag. 318-320
  • 2007 - Somatic Embryogenesis From Whole Flowers, Anthers And Ovaries Of Grapevine (vitis Spp.). - Gambino G., (1)Ruffa P., Vallania R., Gribaudo I. - Plant Cell Tissue And Organ Culture (04987J0), volume 90(1) pag. 79-83
  • 2006 - Simultaneous Detection Of Nine Grapevine Viruses By Multiplex Reverse Transcriptionpolymerase Chain Reaction With Coamplification Of A Plant Rna As Internal Control. - Gambino, G. & Gribaudo, I. - Phytopathology (77147J0), volume 96 pag. 1223-1229
  • 2006 - Molecular Characterization Of Grapevine Plants Transformed With GFLV Resistance Genes: II. - Maghuli F., Leopold S., Da Camara Machado A., Borroto Fernandez E.G., Khan M.A., Gambino G., Gribaudo I., Laimer M. - Plant Cell Reports (06015J0), volume 25 pag. 546-553
  • 2006 - Detection And Elimination Of Viruses In Callus, Somatic Embryos And Regenerated Plantlets Of Grapevine. - Gambino, G., Bondaz, J. & Gribaudo, I. - European Journal Of Plant Pathology (10845J0), volume 114 pag. 397-404
  • 2005 - Molecular Characterization Of Grapevine Plants Transformed With GFLV Resistance Genes: I. - Gambino G., Gribaudo I., Leopold S., Schartl A., Laimer M. - Plant Cell Reports (06015J0), volume 24 pag. 655-662
  • 2005 - Genetic Characterization Of Endangered Grape Cultivars Of Reggio Emilia Province. - Boccacci P., Torello Marinoni D., Gambino G., Botta R., Schneider A. - American Journal Of Enology And Viticulture (06974J0), volume 56(4) pag. 411-416
  • 2005 - Molecular Characterization Of Transgenic Grapevine Plants - Gribaudo I., Gambino G., Leopold S., Laimer M. - Acta Horticulturae , volume 689 pag. 485-492
  • 2004 - Somatic Embryogenesis From Grapevine Anthers: The Optimal Developmental Stage For Collecting Explants. - Gribaudo I., Gambino G., Vallania R. - American Journal Of Enology And Viticulture (06974J0), volume 55 pag. 427-430
  • 2004 - Risanamento Di Cloni Di Vite (vitis Vinifera L.) Da Virus Floematici Mediante Embriogenesi Somatica - Bondaz J., Gambino G., Cuozzo D., Gribaudo I. - Petria , volume 14 pag. 99-108
  • 2004 - Cryopreservation Of Vitis Vinifera L. Somatic Embryos By Vitrification Or Encapsulation-dehydration. - Miaja M.l., Vallania R., Gribaudo I., Gambino G. - Acta Horticulturae , volume 663 pag. 599-603
  • 2003 - Ovary As Valuable Explant For Somatic Embryogenesis Induction In Grapes (vitis Spp.) - Martinelli L., Gribaudo I. Semenzato M., Poletti V., Gambino G. - Acta Horticulturae , volume 603 pag. 499-504
  • 2003 - Transformation Of Vitis Vinifera L. Cv Nebbiolo With The Coat Protein Gene Of Grapevine Fanleaf Virus (gflv). - Gribaudo I., Scariot V., Gambino G., Schubert A., Golles R., Laimer M. - Acta Horticulturae , volume 603 pag. 309-314